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hacat cells  (ATCC)
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
Hacat Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
Hacat Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
Cell Viability In Hacat, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hacat cell line  (CLS Cell Lines Service GmbH)
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
Hacat Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hacat cell line  (ATCC)
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ATCC hacat cell line
In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
Hacat Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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press hacat cells  (CLS Cell Lines Service GmbH)
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
Press Hacat Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of HaCaT cells treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.

Journal: iScience

Article Title: G-quartet driven adaptive hydrogels that integrate constitutional dynamics for antibacterial action and tissue repair

doi: 10.1016/j.isci.2026.116107

Figure Lengend Snippet: In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of HaCaT cells treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.

Article Snippet: WRL68 cells were obtained from National Centre for Cell Science (NCCS), Pune, India, and HaCaT cells were obtained from ATCC (Cat# CRL-2404).

Techniques: In Vitro, In Vivo, Wound Healing Assay, Migration, Immunofluorescence, Staining, Control, Comparison